FIG 3.

Infection with cell-associated or cell-free WITO ± DEAE-dextran with different inhibitors. TZM-bl (A and B) or A3R5 (C and D) target cells were infected with cell-associated (A and C) or cell-free (B and D) WITO virus in the presence of different concentrations of inhibitors. All inhibitors were serially diluted 10-fold at various ranges of concentration (PMPA, 0.1 to 100 μM; nevirapine, 0.4 to 400 nM; T-20, 0.5 to 500 nM; TAK-779, 1 to 1,000 nM), with the exception of maraviroc, which was serially diluted 20-fold (*, 0.015625 to 2,500 nM). Background was subtracted from results, and results were normalized to the DMSO-positive control and summarized as infection based on RLU (A and B) or percentage of GFP⁺ (Far Red⁻) cells (C and D), where 1 is equal to 100% infection. Results are expressed as dose-response curves illustrating the median at each dose dilution.