Fig. 11.

Orientation analysis of fibers from the mammary gland sample. (a) Combined fluorescence (red) and SHG (green) image. Scale bar: 100 μm. (b) The 3D stack of the marked region in (a). Scale bar: 50 μm. (c) One frame of the $\theta$ orientation map from the 3D stack, calculated by the 3D algorithm. Scale bar: 50 μm. The two regions shown in (c) are zoomed in to show the details of fiber orientation. (d) Original SHG intensity of region 1. (e) $\theta$ orientation map of region 1. Scale bar: 30 μm. The arrows shown in (d) and (e) point out the branched structure. (f) Original SHG intensity of region 2. (g) $\theta$ orientation map of region 2. Scale bar: 15 μm. (h) The distribution histogram of $\theta$ corresponding to this frame.