Table III.

Predicted ΔG_fold for the Series of Proteins Considered in this Work^a

No.	Protein	$-\mathrm{\Delta }{G}_{\text{fold}}^{\text{elec}}$	$-\mathrm{\Delta }{G}_{\text{fold}}^{\text{obs}}$	|Error|	Best εp	Best εeff	$-{\overline{\mathrm{D}\mathrm{G}}}_{\text{fold}}^{\text{calc}}$	|Error|
1	SSO7d	7.9	8.0	0.1	40	40	8.5	0.5
2	Thioredoxinb	4.4	9.0	4.6	40	35	3.1	5.9
3	Barstar	5.9	5.7	0.2	35	35	4.9	0.8
4	Apoflavodoxin	4.3	4.3	0.0	35	40	5.0	0.7
5	λ-Repressor	4.5	4.6	0.1	40	40	4.8	0.2
6	Snase	6.3	6.2	0.1	35	40	8.0	1.8
7	BsHpr, Phosphotransferase	3.1	4.0	0.9	40	35	2.1	1.9
8	FeCyt b562	7.7	5.2	2.5	35	40	9.7	4.5
9	Arc Repressor	4.3	4.6	0.3	40	35	1.7	2.9
10	Aspartate Amilotransferase	26.8	28.9	2.1	40	40	31.0	2.1
11	Chey	11.6	9.5	2.1	35	40	13.5	4.0
12	GDH Domain II	5.6	4.9	0.7	35	35	4.2	0.7
13	Histidine Phosphocarrier	5.4	8.2	2.8	40	35	4.5	3.7
14	Phosphotransferase, Histidine containing protein	5.3	5.2	0.1	40	35	4.0	1.2
15	Ribosomal	7.4	10.7	3.3	40	35	6.4	4.3
16	RNase H*	7.1	7.5	0.4	40	35	6.5	1.0
17	Ferridoxinb	3.8	9.3	5.5	40	35	3.2	6.1
18	O-Methyl Guanine DNA methyltransferase	10.2	10.2	0.0	35	35	9.1	1.1
19	Sac7d	7.8	7.4	0.4	40	40	9.0	1.6
20	Histone	7.8	7.2	0.6	40	35	7.6	0.4
21	PFRD-XC4	2.1	3.2	1.1	40	35	1.2	2.0
22	Staphylococcal Nuclease I92K	1.5	2.3	0.8	40	40	2.8	0.5
23	Staphylococcal Nuclease I92E	3.8	4.2	0.4	40	35	2.2	2
24	Staphylococcal Nuclease	11.5	11.5	0.0	35	35	10.3	1.2
25	Bs CSP	1.1	1.2	0.1	35	35	0.8	0.4
26	Bc CSP	5.2	5.0	0.2	40	35	4.2	0.8
27	Tm CSP	6.8	6.5	0.3	35	40	8.0	1.5
28	Ubiquitin D21N	5.1	6.1	1.0	40	35	3.9	2.2
29	Ubiquitin F45W	6.9	7.4	0.5	40	35	5.9	1.5
30	Ubiquitin K27A	4.3	4.4	0.1	40	40	4.8	0.4
31	Tm DHFR	30.4	30.1	0.3	40	35	25.9	4.2
32	Ec DHFR	6.5	6.0	0.5	35	35	4.9	1.1
33	Ribonuclease	11.2	10.5	0.7	40	40	13.1	2.6
34	Glucanase Cb	0.0	0.0	0.0	–	–	0.0	0.0
35	Phospholipid A2	7.8	6.5	1.3	35	40	9.1	2.6
36	Trypsin Proteinase	7.7	7.5	0.2	35	35	6.8	0.7
37	Interleukin	9.7	9.1	0.6	35	35	8.9	0.2
38	Adhesion transferase Y92E	7.5	8.0	0.5	40	40	8.4	0.4
39	Complement	2.6	2.7	0.1	35	35	2.5	0.2
40	Cytochrome b5 Rat	3.5	3.2	0.3	35	35	2.3	0.9
41	Gene V DNA Binding	7.6	9.0	1.4	40	35	6.6	2.4
42	Glu Transferase	13.6	14.4	0.8	35	35	10.2	4.2
43	Growth Factor	0.0	0.0	–	–	–	0.0	0.0
44	Isomerase	19.2	19.3	0.1	40	35	13.7	5.6
45	Ribosomal S6	11.1	8.0	3.1	35	40	12.9	4.9

^aAll free energies reported are in kcal/mol. The third column reports the experimental values of the absolute stabilities of the tested proteins. The last two columns give the predicted stability according to Eq. (12) and the corresponding error. The sources of the observed values are given in Table I.

^bProteins containing a disulfide bond (which is not treated by the present method) where the corresponding “missing” energy is around 5 kcal/mole.