Table 3.
Rate (%) of CD4 T cell IFNγ and IL-17 antigen-specific positive responses among groups categorized by Chlamydia trachomatis infection and antibody status.
| IFNγ | ||||||
| Antigen | Positive responsea | Active Infection (n = 8) |
Seropositive/ Uninfected (n = 13) |
Seronegative/ Uninfected (n = 18) |
Total | P valueb |
| HSP60 | No | 4 (50.00) | 5 (38.46) | 11(61.11) | 20 (51.28) | 0.451 |
| Yes | 4 (50.00) | 8 (61.54) | 7 (38.89) | 19 (48.72) | ||
| EB | No | 3 (37.50) | 5 (38.46) | 15 (83.33) | 23 (58.97) | 0.019c |
| Yes | 5 (62.50) | 8 (61.54) | 3 (16.67) | 16 (41.03) | ||
| PmpG | No | 6 (75.00) | 5 (38.46) | 11 (61.11) | 22 (56.41) | 0.253 |
| Yes | 2 (25.00) | 8 (61.54) | 7 (38.89) | 17 (43.59) | ||
| PmpF | No | 5 (62.50) | 4 (30.77) | 14 (77.78) | 23 (58.97) | 0.032d |
| Yes | 3 (37.50) | 9 (69.23) | 4 (22.22) | 16 (41.03) | ||
| MOMP | No | 6 (75.00) | 7 (53.85) | 17 (94.44) | 30 (76.92) | 0.024e |
| Yes | 2 (25.00) | 6 (46.15) | 1 (5.56) | 9 (23.08) | ||
| IL-17 | ||||||
| Antigen | Positive responsea | Active Infection (n = 8) |
Seropositive/ Uninfected (n = 12) |
Seronegative/ Uninfected (n = 18) |
Total | P valueb |
| HSP60 | No | 7 (87.50) | 10 (76.92) | 16 (88.89) | 33 (84.62) | 0.844 |
| Yes | 1 (12.50) | 3 (23.08) | 2 (11.11) | 6 (15.38) | ||
| EB | No | 6 (75.00) | 10 (76.92) | 16 (88.89) | 32 (82.05) | 0.639 |
| Yes | 2 (25.00) | 3 (23.08) | 2 (11.11) | 7 (17.95) | ||
| PmpG | No | 7 (87.50) | 11 (84.62) | 16 (88.89) | 34 (87.18) | 1.000 |
| Yes | 1 (12.50) | 2 (15.38) | 2 (11.11) | 5 (12.82) | ||
| PmpF | No | 6 (75.00) | 10 (76.92) | 16 (88.89) | 32 (82.05) | 0.639 |
| Yes | 2 (25.00) | 3 (23.08) | 2 (11.11) | 7 (17.95) | ||
| MOMP | No | 6 (75.00) | 11 (84.62) | 16 (88.89) | 33 (84.62) | 0.737 |
| Yes | 2 (25.00) | 2 (15.38) | 2 (11.11) | 6 (15.38) | ||
A positive response was defined by a net count ≥25 and at least three-fold higher than the media response.
P values were calculated using Fisher’s exact test.
For the pairwise comparisons of IFN-γ EB, the Seropositive/Uninfected group and the Seronegative/Uninfected group were significantly different (P = 0.021), but neither of the other two comparisons was significant (P = 1.00 for Active Infection versus Seropositive/Uninfected and P = 0.060 for Active Infection versus Seronegative/Uninfected).
For the pairwise comparisons of IFN-γ PmpF, the Seropositive/Uninfected group and the Seronegative/Uninfected group were significantly different (P = 0.013), but neither of the other two comparisons was significant (P = 0.203 for Active Infection versus Seropositive/Uninfected and P = 0.635 for Active Infection versus Seronegative/Uninfected).
For the pairwise comparisons of IFN-γ MOMP, the Seropositive/Uninfected group and the Seronegative/Uninfected group were again significantly different (P = 0.012), but neither of the other two comparisons was significant (P = 0.399 for Active Infection versus Seropositive/Uninfected and P = 0.215 for Active Infection versus Seronegative/Uninfected).