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. Author manuscript; available in PMC: 2015 Jul 17.
Published in final edited form as: Cell. 2015 Apr 23;161(3):459–469. doi: 10.1016/j.cell.2015.03.051

Figure 1. Heteroplasmy shift in NZB/BALB MII oocytes using mito-ApaLI.

Figure 1

(A) Injection of mito-ApaLI mRNA in oocytes for induction of heteroplasmy shift.

(B) Mitochondrial co-localization of mito-GFP and mito-ApaLI with Mitotracker in injected oocytes by immunofluorescence. Scale bars, 10μm.

(C) RFLP analysis and quantification of mtDNA heteroplasmy in control and mito-ApaLI injected MII oocytes after 48 h (Control n=16; mito-ApaLI n=12). Representative gel.

(D) Quantification of mtDNA copy number by qPCR in control and mito-ApaLI injected oocytes MII after 48 h (Control n=12; mito-ApaLI n=12).

Error bars represent ± SEM. ****p<0.0001. See also Figure S1.