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. Author manuscript; available in PMC: 2015 Jul 17.
Published in final edited form as: Cell. 2015 Apr 23;161(3):459–469. doi: 10.1016/j.cell.2015.03.051

Figure 2. Heteroplasmy shift in NZB/BALB embryos using mito-ApaLI.

Figure 2

(A) Injection of mito-ApaLI mRNA in one-cell embryos for induction of heteroplasmy shift.

(B) In vitro development of mito-ApaLI injected embryos to blastocyst stage. Time-lapse images of EGFP reporter expression at different developmental stages.

(C) RFLP analysis and quantification of mtDNA heteroplasmy in control and mito-ApaLI injected embryos (Control n=10; mito-ApaLI n=8). Representative gel.

(D) Quantification of mtDNA copy number by qPCR in control and mito-ApaLI injected embryos (Control n=18; mito-ApaLI n=12).

Error bars represent ± SEM. ***p<0.001. ****p<0.0001. See also Figure S2.