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. 2015 Jul 17;10(7):e0133353. doi: 10.1371/journal.pone.0133353

Fig 3. Dlg1AB selectively enhances NFAT-dependent transcription of IFNγ and TNFα but not IL-2 or granzyme B.

Fig 3

(A-C) Purified primary OT-1 CD8+ T cells were stimulated with anti-CD3/anti-CD28 followed by infection with control (Empty) or Dlg1-viruses. (A) Cells were isolated and assessed for Dlg1 overexpression via qPCR. (B-C) Cells were restimulated with anti-CD3/anti-CD28 or left unstimulated in the presence or absence of 10μM p38 inhibitor (SB203580). RNA was isolated for qPCR analysis. mRNA was normalized to L32 and fold-increase in mRNA expression vs. unstimulated samples is shown. Error bars represent SD of samples analyzed in triplicate. *p < 0.05. Data are representative of three independent experiments. (D) CD8+ OT-1 hybridoma T cells infected with Dlg1 re-expression (bold) and/or Dlg1 knockdown (KD) constructs where stimulated with anti-CD3/anti-CD28 or left unstimulated. RNA was isolated for qPCR analysis. mRNA was normalized to L32 and fold-increase in mRNA expression vs. unstimulated samples is shown. Error bars represent SD of samples analyzed in triplicate.