Fig 7. PleC kinase conformation is required to establish replicative asymmetry.

Concentration gradients are color coded as in Fig 5. (A) In ΔpleC mutant cells (K^-P^-B^-), free active form of DivL is lower than in wild-type cells, resulting in loss of CtrA~P in the predivisional cell. (B) In pleC _H610A mutant cells (K^-P^-B⁺), an elevated level of DivK~P results in less active form of DivL and reduced CtrA~P. (C) In pleC _F778L mutant cells (K^-P⁺B⁺), inhibitor sequestration is retained, resulting in a normal CtrA~P gradient.