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. 2015 Jul 17;10(7):e0133450. doi: 10.1371/journal.pone.0133450

Fig 1. Establishment of IRF7 overexpression and knockdown DF-1 cell lines.

Fig 1

(A) Multiple sequence alignment of IRF7 sequences in exon 8 nucleotide position 169 to 221. Molecular cloned IRF7 clone (accession) and the NCBI reference sequence (NM_205372) were aligned against the reference chicken genome (galGal4). (B) Schematic diagram of overexpression (up) and knockdown (down) PiggyBac transposon expression vectors. (C) Related quantitation of IRF7 in control (Vector) and overexpression (CMV-IRF7) cell lines. (D) Knockdown efficiency of each siRNA for IRF7 in the overexpressed cell line. siRNA non-specific (NS) to the chicken genome was used as a control. (E) Knockdown efficiency of IRF7 in the knockdown cell line (KD) as compared to control cells (NS). * P < 0.05, ** P < 0.01, *** P <0.001. Error bars indicate the SEM of triplicate analyses.