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. 2015 Jul 17;10(7):e0132332. doi: 10.1371/journal.pone.0132332

Fig 1. Schematic drawing of the luciferase reporter vectors (psi-CHECK2) contains NBS1 3’-UTR sequence with either the G or C at the rs2735383 locus.

Fig 1

The luciferase activity of the rs2735383 variant allele on luciferase reporter bearing NBS1 3’-UTR co-transfected with or without hsa-miR-509-5p mimics or hsa-miR-509-5p inhibitors in HCT116 cells (A) and HT-29 cells (B). Renilla luciferase activity was measured and normalized to firefly luciferase. Six replicates were carried out for each group, and the experiment was repeated at least three times. Data are mean±standard error of the mean; **P<0.01. (C) NBS1 mRNA expression level in tumor tissue samples from colorectal cancer individuals with different rs2735383 variant alleles genotype (11 rs2735383GG, 13 rs2735383GC and 5 rs2735383CC); data are mean±standard error of the mean, normalized to GAPDH, P = 0.027. (D) hsa-miR-509-5p mRNA expression in 29 colorectal cancer tissues grouped by rs2735383 C/G genotypes. The hsa-miR-509-5p mRNA expression was calculated relative to expression of U6 mRNA. Data are mean ± standard error of the mean, P = 0.345.