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. Author manuscript; available in PMC: 2015 Jul 17.
Published in final edited form as: Br J Haematol. 2012 Nov 15;160(2):177–187. doi: 10.1111/bjh.12103

Fig. 2.

Fig. 2

JAK2 is present in fractions containing detergent resistant membranes. (A) SET-2 cell lysates were analysed by density buoyant gradient fractionation. Fractions were removed from the top of the gradient and thus lower fraction numbers correspond to lower buoyant density fractions. Each fraction was analysed by dot blot for GM1 ganglioside, a resident raft lipid, using CTB as a probe. Separation of GM1 into lower and higher buoyant fractions suggests separation of DRMs. Highest detection of GM1 is present in fraction 2, thus designating fraction 2 as the raft fraction. (B) Immunoblot analyses of gradient fractions of untreated cells to detect JAK2 or the resident lipid raft marker, Flotillin-1. (C) Immunoblot analyses for JAK2 and Flotillin-1 as in (B), but utilizing MBCD-treated SET-2 cells. MBCD treatment redistributed JAK2 and Flotillin-1 to the higher density non-raft fractions. Molecular weights are indicated in kDa.