Fig 1. AAM-B is required for HCV propagation.

(A) Huh7.5 cells were transfected with 20 nM of scrambled siRNA (Negative) or the indicated AAM-B specific siRNA constructs for 48 h and then infected with Jc1 for 4 h. At 48 h postinfection, AAM-B mRNA levels were analyzed by qPCR. (B) Using total RNAs isolated from (A), intracellular HCV RNA levels were quantified by qPCR. (C) Naïve Huh7.5 cells were infected with Jc1 harvested from the culture supernatants of (A). At 48 h postinfection, intracellular HCV RNA levels were quantified by qPCR. (D) (Left) AAM-B stable cells were transfected with the indicated siRNAs for 48 h and then infected with Jc1 for 4 h. At 48 h postinfection, total cell lysates were immunoblotted with the indicated antibodies. (Right) The band intensities of viral proteins were quantified using ImageJ software and were expressed as relative fold from the negative control. (E) (Left) Naïve Huh7.5 cells were infected with Jc1 harvested from culture supernatants of (D). At 48 h postinfection, total cell lysates were immunoblotted with the indicated antibodies. (Right) The band intensities of viral proteins were determined by using ImageJ software and were shown as relative fold from the negative control.