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. 2015 Jul 17;10(7):e0132839. doi: 10.1371/journal.pone.0132839

Fig 2. AAM-B is not required in the replication step of the HCV life cycle.

Fig 2

Huh7 cells harboring HCV subgenomic replicon derived from genotype 1b were transfected with the indicated siRNAs for 72 h. Both AAM-B mRNA level (A) and intracellular HCV RNA level (B) were quantified by qPCR. (C) Protein expression levels were analyzed by immunoblot analysis using the indicated antibodies. (D) Huh6 cells harboring HCV subgenomic replicon derived from genotype 2a were transfected with the indicated siRNAs for 72 h. Both AAM-B mRNA level (D) and intracellular HCV RNA level (E) were quantified by qPCR. The asterisks indicate significant difference (**, p<0.01) from the value for the negative control. (F) Protein expression levels in HCV replicon cells of genotype 2a were analyzed by immunoblotting with the indicated antibodies.