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. 2015 Jul 17;10(7):e0132839. doi: 10.1371/journal.pone.0132839

Fig 3. AAM-B is involved in the virion assembly and release stage of the HCV life cycle.

Fig 3

(A, B) Huh7.5 cells were electroporated with in vitro transcribed Jc1 RNA for 24 h and then transfected with the indicated siRNAs. At 48 h after transfection, both AAM-B mRNA level (A) and HCV RNA levels (B) were quantified by qPCR. (C) Naïve Huh7.5 cells were infected with culture supernatant harvested from (A). At 48 h postinfection, intracellular HCV RNA level was quantified by qPCR. (D) AAM-B stable cells were infected with Jc1 for 4 h. At 48 h postinfection, the cells were transfected with the indicated siRNAs. At 48 h after siRNA transfection, both AAM-B mRNA level (D) and HCV RNA levels (E) were quantified by qPCR. (F) Naïve Huh7 cells were infected with culture supernatant harvested from (D). At 48 h postinfection, intracellular HCV RNA level was quantified by qPCR. The asterisks indicate significant differences (*, p<0.05;**, p<0.01) from the value for the negative control.