FIGURE 6.

Higher expression of proinflammatory mediators from CRB- and BS-infiltrating neutrophils from LysMCre-SOCS3^fl/fl mice with atypical EAE. (A) CD45⁺CD11b⁺Ly-6C^lowLy-6G⁺ neutrophils were sorted from CRB- and BS-infiltrating mononuclear cells at the peak of disease from LysMCre-SOCS3^fl/fl mice with atypical EAE or from SOCS3^fl/fl mice with classical EAE, mRNA isolated, and analyzed for CXCL2, CXCL10, iNOS, IL-1β, and TNF-α. Mean ± SD. Fold induction of indicated genes was compared between SOCS3^fl/fl mice (n = 5) and LysMCre-SOCS3^fl/fl mice (n = 4). Data represent three individual experiments. CD45⁺CD11b⁺Ly-6C^lowLy-6G⁺ neutrophils were sorted from BM, blood, and SP of LysMCre-SOCS3^fl/fl mice with atypical EAE or from SOCS3^fl/fl mice with classical EAE at the indicated disease stages, mRNA isolated, and analyzed for CCL2, TNF-α (B), iNOS (C), IL-1β, CXCL2, and CXCL10 (D). Mean ± SD (n = 4 mice). Data represent fold induction compared with naive SOCS3^fl/fl BM (n = 3 mice in each group). *p < 0.05, **p < 0.01.