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. 2015 Jun 19;195(3):882–891. doi: 10.4049/jimmunol.1403110

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Copyright © 2015 The Authors

This is an open-access article distributed under the terms of the CC-BY 3.0 Unported license.

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FIGURE 3. — M. tuberculosis–driven collagen degradation is MT1-MMP–dependent. (A and B) Human monocytes were seeded on FITC-conjugated type I collagen and infected with M. tuberculosis. Collagen degradation was analyzed by immunofluorescent microscopy. Blue is DAPI nuclear stain; green is type I collagen. M. tuberculosis infection causes greater collagen degradation. (C) MT1-MMP activity was neutralized with an inhibitory anti-MT1-MMP Ab (MAB3328, Millipore) at 10 μg/ml. MT1-MMP inhibition inhibited areas of collagen breakdown. (D) An isotype control Ab did not inhibit collagen degradation. (E) Fluorescence quantitation confirms collagen degradation is MT1-MMP–dependent. Data are from a single experiment and are representative of three independent experiments. Mean values ± SD are shown. ***p < 0.001 by one-way ANOVA with a Tukey post hoc test.