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. 2015 Jul 17;10(7):e0131466. doi: 10.1371/journal.pone.0131466

Fig 6. Specific detection of Salmonella enterica Typhimurium LT2 with a P22 phagosensor.

Fig 6

(A) Schematic representation of the P22 construct. A PCR fragment containing the gfpmut2 gene under the control of PrrnB promoter (red boxes), the kanamycin resistance-cassette and 80 bp homologous to the insertion region (dark and light blue) was recombined into the P22 gp45/gp46 intergenic region. (B) A culture of S. enterica Typhimurium LT2 was either infected with the P22::PrrnB-gfp phage (green curve) or non infected (red curve) then incubated at 30°C for 1 hour. Average fluorescent cells number was obtained from triplicate samples. (C) Optical (upper) and fluorescence (lower) microscope images of S. enterica Typhimurium LT2 incubated at 30°C for 1 h with P22::PrrnB-gfp.