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. 2015 Jul 17;10(7):e0133405. doi: 10.1371/journal.pone.0133405

Fig 7. Effect of JYCF and fluoxetine on the levels of brain monoamine neurotransmitters and metabolic products in mice.

Fig 7

(A) The levels of 5-HT in the hippocampus and prefrontal cortex. In the hippocampus, one-way ANOVA indicated a significant effect [F (5, 42) = 3.037, P < 0.05] of treatment with JYCF and fluoxetine on 5-HT concentration. JYCF (2.5 g/kg) and fluoxetine (20 mg/kg) significantly increased 5-HT levels compared to the CUMS group (P < 0.05). In the prefrontal cortex, one-way ANOVA indicated no significant effect [F (5, 42) = 2.347, P > 0.05]. Results are expressed as mean ± S.E.M. *P<0.05, **P < 0.01, as compared with the control group; #P < 0.05, ##P < 0.01, as compared with the CUMS group. (B) The levels of NE in the hippocampus and prefrontal cortex. In the prefrontal cortex, a one-way ANOVA revealed a significant effect [F (5, 42) = 3.125, P < 0.05] of treatment with JYCF and fluoxetine on NE concentration. JYCF (1.25, 2.5, 5g/kg) and fluoxetine (20 mg/kg) significantly increased NE level compared to the CUMS group (P < 0.01, P < 0.01, P < 0.05, P< 0.05 respectively). In the hippocampus, one-way ANOVA indicated no significant effect [F (5, 42) = 0.359, P > 0.05]. Results are expressed as mean ± S.E.M. *P<0.05, **P < 0.01, as compared with the control group; #P < 0.05, ##P < 0.01, as compared with the CUMS group. (C) The levels of HVA in the hippocampus and prefrontal cortex. In the hippocampus, a one-way ANOVA indicated significantly difference after administration of JYCF and fluoxetine in CUMS mice on the concentration of HVA [F (5, 42) = 4.070, P < 0.01], JYCF (1.25, 2.5, 5 g/kg) did not reverse the reduction of HVA (P > 0.05). In the prefrontal cortex, one-way ANOVA indicated no significant effect [F (5, 42) = 0.411, P > 0.05]. Results are expressed as mean ± S.E.M. *P < 0.05, **P< 0.01, as compared with the control group; #P < 0.05, ##P < 0.01, as compared with the CUMS group. (D) The levels of 5-HIAA in the hippocampus and prefrontal cortex. In the hippocampus, a one-way ANOVA indicated significantly difference after administration of JYCF and fluoxetine in CUMS mice on the concentration of 5-HIAA [F (5, 42) = 9.783, P < 0.01], JYCF (1.25, 2.5, 5 g/kg) did not reverse the reduction of 5-HIAA (P > 0.05). In the prefrontal cortex,one-way ANOVA indicated no significant effect [F (5, 42) = 0.740, P > 0.05]. Results are expressed as mean ± S.E.M. *P < 0.05, **P < 0.01, as compared with the control group; #P < 0.05, ##P < 0.01, as compared with the CUMS group. (E) The levels of DOPAC in the hippocampus and prefrontal cortex. In the hippocampus, a one-way ANOVA indicated significantly difference after administration of JYCF and fluoxetine in CUMS mice on DOPAC [F (5, 42) = 3.291, P < 0.05], JYCF (1.25, 2.5, 5 g/kg) did not reverse the reduction of DOPAC (P > 0.05). In the prefrontal cortex, one-way ANOVA indicated no significant effect [F (5, 42) = 0.530, P > 0.05]. Results are expressed as mean ± S.E.M. *P < 0.05, **P < 0.01, as compared with the control group; #P < 0.05, ##P < 0.01, as compared with the CUMS group.