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. 2015 Jul 17;10(7):e0133399. doi: 10.1371/journal.pone.0133399

Fig 1. Cell spreading properties depend on cell passage number.

Fig 1

RbCF2 and RbCF8 cells were serum starved for 48 h, trypsinized and then replated in presence of 10% serum for different post-plating times. Cells were fixed and cell morphology was analyzed. (A) Representative bright field images of RbCF2 and RbCF8 cells. (B) Graphs represent time-related changes in cell spreading behavior and (D) cell polarization. An average of 70 RbCF8 cells and 154 RbCF2 cells were analyzed. (C) Representative images of RbCF2 and RbCF8 cells at different times after plating and stained with phalloidin (red) and DAPI (blue). Scale bar = 35 μm at 30X magnification.