Fig 2. Distribution of vimentin IFs controls filament rearrangement during cell spreading.

RbCF2 and RbCF8 cells were cultured as in Fig 1 and plated for 30 min followed by treatment in presence or absence of WFA (1 μM) for different time points. Cells were fixed and stained (A) vimentin (green) and DAPI (blue) or (B-C) with pSer38vim (pSer38vim, green) and DAPI (blue). Scale bar = 35 μm. Insert panels in panels B and C represent 60X magnification of selected areas. Scale bar = 20 μm.