Fig 3. Mature focal adhesions require coalescence with vimentin and develop in a cell-passage dependent manner.

(A) RbCF2 and RbCF8 cells were cultured as in Fig 1, plated for 30 min followed by treatment in presence or absence of WFA (1 μM) for 1 h. Cells were fixed and stained with paxillin (green), vimentin (red), and DAPI (blue). Scale bar = 35 μm. (B) Peripheral FA mean areas from RbCF2 (black columns) and RbCF8 (grey columns) cells were measured and represented in arbitrary units. (*P <0.0001; **P <0.0001).