Fig 4. Soluble vimentin is targeted by WFA when overexpressed in RbCF8 cells under myosin perturbation.

RbCF8 cells were transiently transfected with human vimentin-GFP cDNA. Two-days post transfection cells were treated with blebbistatin (Blebb, 50 μM) for 2 h (A), washed and then treated in presence (B) or absence of 500 nM WFA (C) for 18 h. Endogenous (red) and exogenous (green; GFP) expression of vimentin was assessed by immunohistochemistry. Scale bar = 35 μm at 30X magnification. Insert panels represent 100X magnification of selected areas. Scale bar = 100 μm.