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. 2015 Jul 17;10(7):e0132977. doi: 10.1371/journal.pone.0132977

Fig 1. BORIS expression and SP analysis in human epithelial tumor cell lines.

Fig 1

(A) BORIS expression in human tumor cell lines. Total RNA from NCCIT (embryonic), HeLa (cervical), HT29 (colon), MCF7 (non-invasive breast) and MDA-MB-231 (invasive breast) tumor cells were extracted and BORIS expression was analyzed by qRT-PCR. The results were normalized to GAPDH and related to NCCIT cells. Error bars represent the mean ± SD (n = 3). (B) BORIS expression as detected using BORIS-MB. Representative images of HeLa, HT29, MCF7 and MB-MDA 231 cells, 40X magnification. Cells were incubated with Cy3-BORIS MB (200 nM) and Hoechst 33342 (5 μg/mL) at 37°C for 1.5 hour in serum-free medium and then examined under fluorescent microscopy. White arrows indicate Hoechst negative cells which are also BORIS mRNA positive as detected by BORIS-MB.