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. 2015 Jul 17;10(7):e0132977. doi: 10.1371/journal.pone.0132977

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© 2015 Alberti et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — Cells were seeded at low density (1,000 cells/ml) in sphere culture medium in low attachment plates. After 10–15 days spheres were collected for RNA extraction and an aliquot of spheres was seeded in normal medium with serum to allow the differentiation (differentiated-spheres). BORIS expression was analyzed by qRT PCR in (A) colon-spheres (HT29 spheres) and differentiated-spheres (HT29 DIFF spheres) and in (B) mammo-spheres (MCF7 spheres) and differentiated-spheres (MCF7 DIFF spheres). Data were normalized to GAPDH and related to parental cells (cells). One representative experiment of 4 independent experiments is shown. Asterisks indicate statistically significant difference (p<0.05) between spheres and parental cells. (C) Representative images of colon-spheres and mammo-spheres are shown, 4X magnification. Black scale bars indicate 250 μm.