Figure 8. Effects of MPC1 or MPC2 expression on epicatechin (EPI) stimulated C2C12 myoblast respiration.
MPC1 or MPC2 expression, measured by RT PCR was significantly repressed using lentiviral shRNAs in C2C12 myoblasts (inset). Rates of maximal uncoupler-stimulated respiration in intact myoblasts incubated in unbuffered DMEM containing pyruvate was measured in control, MPC1, or MPC2 knockdown cells (n=6).