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. 2015 Jul 17;10(7):e0133038. doi: 10.1371/journal.pone.0133038

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© 2015 Ayllón et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) I. scapularis ISE6 cells were treated with Tudor-SN or Rs86 control dsRNAs and infected with A. phagocytophilum NY18. A. phagocytophilum DNA levels were characterized by msp4 real-time PCR normalizing against tick 16S rDNA. Normalized Ct values were compared between groups by Student's t-test with unequal variance and were not statistically different between Tudor-SN and Rs86 dsRNA-treated cells (P = 0.05; N = 4 wells per treatment). (B) I. scapularis female ticks were injected with Tudor-SN or Rs86 control dsRNAs and infected with A. phagocytophilum NY18 by feeding on an infected sheep. A. phagocytophilum DNA levels were characterized in tick guts and salivary glands by msp4 real-time PCR normalizing against tick 16S rDNA. Normalized Ct values were compared between groups by Student's t-test with unequal variance and were not statistically different between Tudor-SN and Rs86 dsRNA-injected ticks (P = 0.05; N = 10 ticks per group).