. 2015 Jul 17;10(7):e0133038. doi: 10.1371/journal.pone.0133038

Table 1. Role of Tudor-SN in tick dsRNA-mediated RNAi.

Experimental group (injected dsRNA)	Tudor-SN / AgoA expression silencing (%)	SUB expression silencing (%)
Tudor-SN + SUB	89±13^a, 91±17^b	NS^a,b
Tudor-SN	65±36^a, 62±19^b	NS^a,b
SUB	NS for AgoA and Tudor-SN ^a,b	67±8^a, 69±5^b

Ticks were injected with dsRNA targeting Tudor-SN (^aTudor or ^bSN domain) and SUB to see the effect of Tudor-SN gene knockdown on SUB expression. Gene knockdown was evaluated with respect to Rs86 control. Statistical analysis was conducted with normalized Ct values between test and control groups by Students t-test. Only significant values (P<0.05) are shown. Two independent experiments with 20 ticks each were conducted. Values are shown as Ave±SD (number of independent experimental replicates (N) = 20). Abbreviation: NS, not significant.