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. 2015 Jul 17;10(7):e0133220. doi: 10.1371/journal.pone.0133220

Fig 3. Molecular characterization of novel M1V RAG1 mutation.

Fig 3

(A) Gene expression of RAG1 in HEK293 cells transfected with wild-type RAG1 and M1V RAG1 mutant by quantitative real time PCR. Cells transfected with mock vector served as a control. Wild type expression was normalized to 1 and percentage of RAG1 M1V gene expression was calculated. (B) Protein expression of RAG1 in HEK293 cells transfected with wild-type RAG1 and M1V RAG1 mutant by western blot. Cells transfected with mock vector served as a control. The arrow indicates RAG1 protein as identified through its molecular weight of 130kD.