Table 1. In vitro immunoglobulin production and ³H-Thymidin incorporation of PBMCs and lymphoblastoid cells.

immunoglobulin production
in vitro (ng/ml)
		control			patient
		mean		SD	mean		SD
unstimulated	IgG	89	±	37	n.a.
	IgM	103	±	53	n.a.
EBV transformed	IgG	6388	±	4239	887	±	76
	IgM	24078	±	7946	38127	±	1805
3H Thymidin incorporation
dpm
unstimulated		1920	±	783	n.a.
EBV transformed		75979	±	2795	90914	±	305

Unstimulated peripheral blood mononuclear cells and EBV-transformed lymphoblastoid cells were incubated for 8 days and the supernatant IgM/G concentration was determined by ELISA. Additionally the ³H-thymidin incorporation was determined. Results are expressed as mean and standard deviation (control unstimulated IgM/G: n = 9; control EBV-transformed IgM/G: n = 3; patient EBV-transformed IgM/G: n = 1 (triplicate); control unstimulated ³H-thymidin incorporation: n = 9; control EBV-transformed ³H-thymidin incorporation: n = 1 (triplicate); patient EBV-transformed ³H-thymidin incorporation: n = 1 (triplicate)).