Fig 4.

An example of competition binding assays with 4313, 4325 and 4337 conserved HABP analogues (AMA-1 derived) [74]. Original radioisotope-labelled peptide-specific binding inhibited by analogous peptide is shown at 100 and 800 nM. The inhibition assay was performed with original non-labelled peptides and their analogous peptides. The asterisk (*) represents original peptides' specific binding. Underlined residues corresponding to the critical binding residues, whose modifications were analysed at 10, 100 and 800 nM concentrations (only two are shown in the figure), displayed the same reduced RBC binding pattern (≥50%) when modified.