Table 2.

Amino acid sequences of some representative native and modified HABPs (in bold) derived from MSP-1 [81, 82, 84], EBA-175 [85], SERA [90], RESA-155 [89], MSP-2 [86] and AMA-1 [83, 87, 88] proteins. The letters a and b represent repetitions of these experiments in different groups of Aotus monkeys. Sequences have been aligned according to the correct identification of residues corresponding to modified HABP HLADR²1* binding motif and reading register in Pockets 1, 4, 6 and 9 [24, 91, 94]. Antibody titres were determined by IFA (shown in brackets) corresponding to sera dilution and the number of Aotus that developed such antibodies previous to challenge. Each group of monkeys received two or three doses of modified HABP. Prot. = the number of monkeys which were fully protected against intravenous inoculation of 100,000 infected erythrocytes from the P. falciparum Aotus adapted FVO strain. Parasitaemia was determined by the very sensitive Acridine Orange methodology.