Fig 2.

Homocysteine releases Ca²⁺ from 2,5 di-(tertbutyl)-1,4-hydroquinone (TBHQ)– insensitive stores in platelets from healthy and diabetic donors. Human platelets from healthy individuals (A, C and E) and diabetic patients (B, D and F) were suspended in a Ca²⁺-free medium (100 μm EGTA was added). Cells were treated with 20 μm TBHQ and 4 min. later homocysteine (Hcy; 50, 100 or 200 μm) was added, as indicated. Traces are representative of 10–21 independent experiments. (G) Values represent Hcy-induced Ca²⁺ release estimated as described in Material and methods. P < 0.05 compared to Hcy-evoked Ca²⁺ release in platelets from healthy donors.