Fig 3.

MS analysis of spot 1 excised from 2-DE gels obtained from RCL2P and frozen tissues respectively were presented on panel A, and the two subsequent MS/MS analysis of the 1783.92 kD protein peak are shown on panel B. Via Swiss-Prot database searching the Heat Shock protein β-1 (accession number P04792, theoretical pl value 5.98 and theoretical Mr 22826 Da) was identified. Mascott scores obtained were 74 and 71 for protein extracted from RCL2P and Frozen maps, respectively and the sequence coverage were 54 and 76%. The two MS/MS spectra of m/z 1783.92 generated a partial y ion series which led to the identification via Swiss-Prot database search of VSLDVNHFAPDELTVK from Heat Shock protein beta-1.