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Western blot performed using antibodies against E-cadherin, Sp1, α-tubulin, phospho MEK 1/2, phospho-p42 and phospho-p44 MAPK using protocol A extraction on two colonic mucosa tissue samples (A). Western blot performed using Sp1 and α-tubulin antibodies on proteins extracted using protocol D, compared to protocol A, of one colonic mucosa tissue sample (B).
