Table 1.
PCR primers and protocols
| Genes | Primers | PCR protocol |
|---|---|---|
| ANP | Up: 5′- ATG GGC TCC TTC TCC ATC ACC -3′ | 94°C 30 sec, 58°C 30 sec. 72°C 1 min. |
| Down: 5′- GTA CCG GAA GCT GTT GCA GCC -3′ | 35 cycles | |
| VEGF | Up: 5′- CCAGCACATAGGAGAGATGAGCTTC -3′ | 94°C 20 sec, 55°C 30 sec. 72°C 1 min. |
| Down: 5′- GGTGTGGTGGTGACATGGTTAATC -3′ | 30 cycles | |
| b-FGF | Up: 5′- ACACGTCAAACTACAACTCCA -3′ | 94°C 15sec, 55°C 15sec. 72°C 30 sec. |
| Down: 5′- TCAGCTCTTAGCA GACATTGG -3′ | 35 cycles | |
| TGF-β | Up: 5′- CTAAGGTGGACCGCAACAAC -3′ | 94°C 15sec, 55°C 15sec. 72°C 30 sec. |
| Down: 5′- CGGTTCATGTCATGGATGGG TG -3′ | 35 cycles | |
| Ang-2 | Up: 5′- GCAACGAGTT TGTCTC -3′ | 94°C 45 sec, 55°C 45 sec. 72°C 1 min. |
| Down: 5′- ACTTTATTCGTATTCTGCTTT -3′ | 35 cycles | |
| β-actin | Up: 5′-GCCATGTACGTAGCCATCCA -3′ | 94°C 20 sec, 55°C 30 sec. 72°C 1 min. |
| Down: 5′-GAACCGCTCATTGCCGATAG -3′ | 30 cycles |