Table 1.
Primary and secondary antibodies used for immunostaining and Western blotting
| Primary antibodies | |||
|---|---|---|---|
| Antigen | Isotype | Dilution | Origin |
| αL(CD11a) | Mouse | 1:50 IH | Instruchemie, Hilversum, The Netherlands |
| αv(CD51) | Arm. hamster | 1:250 WB | Pharmingen, San Diego, CA |
| α6; C28 (CD49f) | Arm. hamster | 1:10 IH 1:60 WB | Kind gift Dr. Wijnands, NKI Amsterdam, The Netherlands |
| β1 (CD29) | Arm. hamster | 1:200 IH 1:1000 WB | Pharmingen |
| β2(CD18) | Mouse | 1:50 IH | Instruchemie |
| β3(CD61) | 1:50 IH | Pharmingen | |
| CD44 | Mouse | 1:1000 IH 1:4000 WB | Pharmingen |
| CD44v6;1.1ASML | Mouse | 1:300 IH 1:800 WB | Kind gift Dr. Sleeman, Institut für Toxikologie und Genetik, Karlsruhe, Germany |
| Ulex Europaeus agglutinin 1 (UEA-1) | Lectin | 1:100 IH | Dakopatts, Glostrup, Denmark |
| UEA-HRP | Lectin | 1:70 IH | Dakopatts |
| Secondary antibodies | |||
|---|---|---|---|
| Antibody | Label | Dilution | Origin |
| Rabbit-anti-mouse IgG | PO | 1:200 IH 1:1000 WB | Dakopatts |
| Goat-anti-Arm. hamster IgG | PO | 1:120 IH 1:500 WB | Jackson, Baltimore, MA |
| Goat-anti-mouse IgG | FITC | 1:100 IH | Jackson |
| Goat-anti-Arm. hamster IgG | FITC | 1:100 IH | Jackson |
| Swine-anti-rabbit IgG | TRITC | 1:40 IH | Dakopatts |
| Rabbit-anti-mouse | TRITC | 1:200 IH | Dakopatts |
| Rabbit-anti-UEA-1 | PO | 1:60 IH | Dakopatts |
Abbreviations: Arm. hamster, Armenian hamster;
UEA-1, Ulex europaeus agglutinin-1;
FITC, fluorescein isothiocyanate;
TRITC, tetramethyl rhodamine isothiocyanate;
IH, immunohistochemistry and cytochemistry;
WB, Western blotting;
PO, peroxidase.