Table 1.
Desensitization rate and equilibrium constants for the DYS background and proline mutants (all rate constants, s−1; n is the number of patches
| Construct | O→D1 | ±SEM | D1→O | ±SEM | E(O↔D1) | ±SEM | n |
|---|---|---|---|---|---|---|---|
| DYS | 101.6 | 35.0 | 18.8 | 5.0 | 6.3 | 2.0 | 4 |
| αP221A | 1101.0 | 126.0 | 17.8 | 5.0 | 74.8 | 26.0 | 3 |
| αP221G | 268.7 | 40.0 | 3.8 | 0.2 | 71.9 | 13.0 | 3 |
| βP232A | 1794.0 | — | 25.0 | — | 71.8 | — | 1 |
| βP232G | 435.7 | 207.0 | 7.4 | 3.0 | 70.6 | 24.0 | 3 |
| δP235A | 303.5 | 94.0 | 3.9 | 2.0 | 84.8 | 15.0 | 2 |
| δP235G | 382.0 | — | 25.0 | — | 15.3 | — | 1 |
| εP230A | 74.7 | 6.0 | 39.0 | 15.0 | 2.4 | 0.7 | 3 |
| εP230G | 213.8 | 63.0 | 14.4 | 4.0 | 16.7 | 4.5 | 3 |
| Construct | D1→D2 | ±SEM | D2→D1 | ±SEM | E(D1↔D2) | ±SEM | n |
| DYS | 3.3 | 1.0 | 0.8 | 0.2 | 5.9 | 3.4 | 4 |
| αP221A | 1.8 | 0.8 | 1.7 | 1.0 | 1.8 | 0.9 | 3 |
| αP221G | 0.9 | 0.0 | 0.6 | 0.3 | 3.9 | 2.7 | 3 |
| βP232A | 1.0 | — | 1.3 | — | 0.8 | — | 1 |
| βP232G | 0.8 | 0.3 | 2.3 | 1.0 | 0.7 | 0.4 | 3 |
| δP235A | 0.7 | 0.1 | 0.3 | 0.2 | 2.2 | 0.2 | 2 |
| δP235G | 6.7 | — | 1.6 | — | 4.2 | — | 1 |
| εP230A | 7.4 | 2.4 | 4.7 | 2.6 | 2.4 | 0.8 | 3 |
| εP230G | 4.1 | 1.3 | 1.0 | 0.4 | 6.5 | 3.3 | 3 |
| Construct | D2→D3 | ±SEM | D3→D2 | ±SEM | E(D2↔D3) | ±SEM | n |
| DYS | 0.33 | 0.26 | 0.110 | 0.090 | 6.7 | 2.7 | 4 |
| αP221A | 0.26 | 0.05 | 0.022 | 0.002 | 11.9 | 3.1 | 3 |
| αP221G | 0.24 | 0.12 | 0.030 | 0.010 | 6.9 | 3.5 | 3 |
| βP232A | 0.13 | — | 0.009 | — | 14.4 | — | 1 |
| βP232G | 0.04 | — | 0.004 | — | 10.0 | — | 1 |
| δP235A | 0.12 | 0.10 | 0.006 | 0.001 | 20.2 | 18.0 | 2 |
| δP235G | 1.10 | — | 0.050 | — | 22.0 | — | 1 |
| εP230A | 1.40 | 0.70 | 0.230 | 0.100 | 6.6 | 1.7 | 3 |
| εP230G | 0.92 | 0.20 | 0.060 | 0.010 | 16.2 | 5.8 | 3 |