Extended Data Figure 5.

D4A inhibits expression of androgen-responsive genes. a, D4A inhibits PSA expression in LAPC4 cells. Cells were treated with DHT (0.5 nM), DHEA (40 nM) or R1881 (0.1 nM) with or without Abi or D4A (1 μM) for 24h. b, D4A inhibits PSA expression in LAPC4 in a dose-dependent manner. c, D4A inhibits AR target gene expression in C4-2 cells. Cells were treated with vehicle control (Ctrl), DHT (0.5 nM) or DHEA (40 nM) with or without Abi (1 μM), D4A (1 μM) or Enz (1 μM) for 24h. d, D4A is comparable to Enz in inhibiting DHT-induced target gene expression in VCaP cells. Gene expression was assessed in triplicate and detected by qPCR and normalized to RPLP0. Experiments were performed with technical replicates (n = 3) and results are shown as mean ± s.d. All experiments were repeatedly independently at least 3 times.