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. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: Neuron. 2015 Jun 25;87(2):341–354. doi: 10.1016/j.neuron.2015.06.007

Figure 3. Airway sensory neuron silencing with QX-314 abolishes capsaicin-induced peptide release and vascular leak.

Figure 3

To assess nociceptor-mediated peptide release capsaicin (1 μmol, intranasal) was administered to naïve mice. BALF CGRP levels were increased one hour following the capsaicin-challenge (A–B). QX-314 (100 μM) treatment 1 hr prior to the capsaicin did not alter capsaicin-induced iCGRP levels (A) but QX-314 (100 μM) when administered immediately after the capsaicin, blocked the CGRP rise (B) (n = 4–15 animals/group; 2–3 cohorts). Reduction in vascular leak (C) following co-administration of capsaicin (1 μmol) with QX-314 (100 μM) (n = 4–5 animals/group; 1 cohort). BALF CGRP levels increased following capsaicin (1 μmol) instillation in OVA-challenged mice (D) and this was blocked by QX-314 pre-treatment one hour before (100 μM). Mean ± S.E.M; Two-tailed unpaired Student’s t-test (n = 5–12 animals/group; 2 cohorts).