Extended Figure 6. Drainage of CSF into the meningeal lymphatic vessels.

a. Representative z-stack of QDot655 filled CSF drainage both in the blood vasculature (sinus) and in the meningeal lymphatic vessels after i.c.v. injection (scale bar = 20 μm). b. Representative images of CD31 and Lyve-1 immunostaining on whole mount meninges. Adult mice were injected i.c.v. with 2.5μg of Alexa 488 conjugated anti-Lyve-1 antibody. Thirty minutes after the injection, the meninges were harvested and stained with CD31. Injected in vivo, the Lyve-1 antibody illuminates the lymphatic vessels (scale bar = 20 μm). c. Representative z-stack of superior sagittal sinus of adult mice injected i.v. with QDot655 and i.c.v. with alexa488 conjugated anti-Lyve-1 antibody. c_i. Coronal section of the z-stack presented in panel c. The signal from the remaining skull and/or collagen-rich structure above the meninges was recorded (blue). c_ii. 3D reconstruction of the z-stack presented in panel c showing the localization of the meningeal lymphatic vessels under the skull (scale bar = 50μm).