Extended Figure 7. Meningeal lymphatic vessels carrying immune cells.

a. Representative images of T cells (CD3e) and lymphatic endothelial cells (Lyve-1) on dural sinuses (scale bar=20μm). a_ii–a_iii. Orthogonal sections representing T cell localization around (a_ii) and within (a_iii) the Lyve-1 structures (scale bar=5μm). b. Quantification of the sinusal T cells and MHCII-expressing cells within the lymphatic vessels (mean ± SEM, n = 7–8 mice). c–d. Representative images of Lyve-1 staining on dural meninges from CD11c^YFP mice (scale bar = 20μm). CD11c-positive cells (most probably dendritic cells) can be found inside the meningeal lymphatic vessels. e. Representative images of B220⁺ cells and lymphatic endothelial cells (Lyve-1) immunolabeling in the meninges (yellow arrowheads indicate B220⁺CD11c^– cells; scale bar=20 μm). f. Representative dot plots of B220⁺ cells (gated on singlets, live, CD45+) within the dural sinuses expressing CD19; ~40% of the B220⁺ cells express CD19.