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To determine if both PMNs and HMVECs contain the PAR-2 receptor isolated PMNs (panel A) and HMVECs (panel B) were lysed, the proteins separated and immunoblotted for the PAR-2 receptor in PMNs and both PAR-1 and PAR2 in HMVECs. PMNs demonstrate immunoreactivity for PAR-2 (Panel A) and HMVECs contain immunoreactivity for both PAR-1 and PAR-2 (panel B). These data represent identical results for two separate experiments.
