Growth of Δ0384 strains in varying CO2 concentrations
Strains harboring the Δ0384 mutation were spotted on to solid medium in triplicate following serial dilutions of cells suspended at an initial OD 730 nm = 0.05 (Tecan). For the single and triple mutants, two biological replicates were spotted on each plate (#1 and #2). Plates were grown in environmental chambers (38°C, 120 μmol photons m−2 s−1) containing different CO2 concentrations (ambient, ~0.04%; enriched, ~0.4%) for 86 h and then photographed.