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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Dev Biol. 2015 Jan 17;408(2):205–212. doi: 10.1016/j.ydbio.2015.01.003

Figure 4. Accumulation of melanophores in growing 15/0 tumor graft.

Figure 4

(A) 15/0 tumor graft heavily infiltrated by host melanophores at 30 days post-transplantation. (B) Ex vivo bright field imaging of a tumor graft showing melanophore (black arrow) accumulation within the 15/0 WT tumor (*; delimited by a dashed white line) compared to normal skin melanophores (blue arrow). (C) One week in vitro culture of tadpole melanophores (m) derived from a semi-solid 15/0 tumor grafted into a LG-15 tadpole recipient that was invaded by melanophores as shown in A. Not the presence of numerous of other cells including 15/0 tumor cells (black arrows) and tumor infiltrating leukocytes (red arrwos) (D) Melanophore (m) enriched three weeks in vitro culture of 15/0 tumor graft-derived melanophores. Note that only a few fibroblast (f) besides melanophores. Animals were imaged using the Nikon SMZ1500 stereomicroscope and cells with an Olympus BX40 microscope.