Figure 4.

The effect of HSP47 on the production of collagen. (A) NIH/3T3 cell was incubated with exogenous TGF-β for 24 h, then transiently transfected with Hsp47 siRNA for another 24 h, scramble siRNA as control, HSP47 and type I collagen protein were determined by Western blot and the densitometry data of Western blot for HSP47 and type I collagen proteins were shown in the column chart. (B) The collagen content in the cell supernatant was measured by Sircol assay. Values are expressed as means ± SEM. *P < 0.05, **P < 0.01 vs. normal control. Experiments were repeated three times