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. 2015 Jun 20;6(8):589–598. doi: 10.1007/s13238-015-0171-3

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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TGF- β increases expression of HSP47 and collagen in a dose-dependent manner. (A) Treatment with TGF-β for 48 h increased the protein level of HSP47 and type I collagen in a dose-dependent manner and densitometric analysis of Western blot for HSP47, COLI were shown in the column chart. RT-PCR revealed increased Hsp47 (B), type I collagen (C), and type III collagen (D) mRNA after treatment with TGF-β for 12 h. Relative mRNA was calculated using a relative ratio to Gapdh. Values are expressed as means ± SEM. *P < 0.05, **P < 0.01 vs. normal control. Experiments were repeated three times