Table I.

Hydrodynamic particle size and surface charge measured for Ag and Ag-gold particles in DI water and culture media.

						Zeta potential (mV)		Effective diameter deff (nm)
		Z-average diameter DLS (nm) in 2 mM NaCl	Effective diameter deff (nm) in DI water	Polydispersity index (PDI)		In 10 mM NaCl	In DI water
Designation	Nanomaterial	NCL	PNNL	NCL	PNNL	NCL	PNNL	In 10%FBS+RPMI
AgAu20NPs	20 nm Ag-gold citrate	24 ± 0.1	29 ± 0.7	0.06	0.124	−48 ± 5	−31 ± 7	52 ± 8
AgAu110NPs	110 nm Ag-gold citrate	104 ± 0.4	110 ± 0.7	0.084	0.106	−43 ± 3.6	−40 ± 2	136 ± 1.8
Agpi20NPs	20 nm Ag citrate	—	29 ± 1	—	0.364	—	−29 ± 1.3	41 ± 0.95
Agpn20NPs	20 nm Ag citrate	—	29.5 ± .5	—	0.18	—	−38 ± 1	57 ± 0.7