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. 2014 Dec 31;39(3):221–229. doi: 10.1016/j.jgr.2014.12.003

Fig. 5.

Fig. 5

The separated minor ginsenoside products of Table I with the silica gel column in the TLC and HPLC. (A) TLC: F2, C-K, C-Mc, C-Y, standard ginsenosides. P, minor ginsenoside product from PPD substrate by the enzyme. Sample 1, product from fractions 1 and 2; Sample 2, C-K from fractions 3–4; Sample 3, from fraction 5; Sample 4, from fraction 6; Sample 5, C-Mc from fraction 7; Sample 6, from fraction 8; Sample 7, C-Y from fraction 9; Sample 8, from fraction 10; and Sample 9, F2 from fractions 10–20. Solvent, chloroform:methanol:water = 7.5:2.5:0.5; 10% H2SO4 as a chromogenic agent. (B) HPLC: The pure Samples 9, 7, 5, and 2 of minor ginsenosides from enzyme reaction in HPLC. Retention time: 45.228 min for Sample 9 (F2); 49.092 min for Sample 5 (C-Mc); 59.395 min for Sample 7 (C-Y); 54.079 min for Sample 2 (C-K). HPLC, high performance liquid chromatography.