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Kinetic parameters of ginsenosidase type-I
| Substrate | Pathway | Km (mM) | Vmax (mM/h) | V01) (mM/h) |
|---|---|---|---|---|
| Rb1 | Hydrolysis of 20-O-Glc of Rb1 to Rd | 16.6 ± 1.6 | 79.6 ± 7.5 | 29.9 |
| Rb2 | Hydrolysis of 3-O-Glc of Rb2 to C-O | 20.4 ± 2.1 | 45.6 ± 4.6 | 15.0 |
| Rc | Hydrolysis of 3-O-Glc of Rc to C-Mc1 | 5.46 ± 0.5 | 6.16 ± 0.6 | 3.98 |
| Rd | Hydrolysis of 3-O-Glc of Rd to F2 | 0.603 ± 0.04 | 1.19 ± 0.11 | 1.12 |
Velocities (V0), in 10mM substrate; using average data of triplicate measurements in the initial reaction time of 5–10 min.
