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. 2015 Jul 3;6:7581. doi: 10.1038/ncomms8581

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(a) Organization of Upf1 and truncated versions (grey arrows) used in this study, Upf1-HD and Upf1. (b) Schematic representation of the RNA used for the magnetic tweezers set-up. (c) Experimental trace showing the activity of Upf1-HD in saturating concentration of ATP. The number of unwound bases is deduced from the molecular extension Z(t) obtained at F=10 pN. From 5,600 to 6,440 s (steps 1–2) the helicase unwound the 156 bp RNA hairpin. From 6,640 to 7,200 s (steps 4–5) the RNA hairpin refolded, while Upf1-HD translocated on ssRNA reaching the 3′ extremity. (d) Distribution of unwinding and translocation rates of Upf1-HD on RNA. (The data corresponds to Upf1 activity observed over 14,400 s, travelling along 2,850 bps in 12 bursts, error bars are s.d.).